Figure 6. Synthesized inhibitors of Cdk4 protect primary sympathetic neurons against NGF deprivation.

Primary cultures of sympathetic neurons (5DIV) were subjected to NGF deprivation in presence and absence of synthesized small molecule inhibitors of Cdk4 for overnight. (A) Representative phase contrast micrographs show retention of neuronal processes even after NGF deprivation in presence of synthesized Cdk4 inhibitors (8A & 8B). (B) Graphical representation of percentage of viable cells following NGF deprivation in presence of synthesized small molecule Cdk4 inhibitors (8A: 5 µM; 8B: 1 µM). Data represented as mean ± SD of two independent experiments performed in duplicates. The asterisks denote statistically significant differences between indicated class: *p<0.05.