Figure 9. Specificity of Cdk4 inhibitors.

(A) Kinase assay of Cdk4 (10 ng) was performed with Cdk4 inhibitors 8A and 8B at different doses as indicated. Data represented as mean ± SEM of 3 independent experiments. The asterisks denote statistically significant differences between indicated classes:*p<0.05. (B) Kinase assay was performed with Cdk4 inhibitors 8A and 8B (10 µM each) with Cdk4, Cdk2 and Cdk5 (10 ng each). Data represented as mean ± STDEV of 2 independent experiments performed in duplicate. The asterisks denote statistically significant differences between indicated classes:*p<0.05. (C) Differentiated PC12 cells were subjected to NGF deprivation in presence or absence inhibitors (8A, 5 µM and 8B, 1 µM) for 8 h. The cells were lysed and equal amount of protein from cell lysate were subjected to immunoprecipitation with Cdk4 antibody. The immunoprecipitated protein was then subjected to kinase assay. Data represented as mean ± SEM of 3 independent experiments. The asterisks denote statistically significant differences between indicated classes:*p<0.05.