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. 2013 Nov 11;8(11):e79062. doi: 10.1371/journal.pone.0079062

Figure 1. GHB has no effect on the holding current of voltage clamped neurons.

Figure 1

A1, Mean holding current over time in response to the application of GHB in VB thalamic neurons (n = 6). Grey area shows SEM. Left inset, representative image of a VB neuron. Scale bar 50 µm. Right inset, voltage response of a representative VB neuron to current injection. Scale bar 20 mV 200 ms. A2, Group results showing the mean holding current before and after GHB. A3, The δ-selective GABAA agonist THIP induced a significant inward current (control: −221±33 pA, THIP: −508±53 pA, n = 6, P = 0.0007). B1, Mean holding current over time in response to the application of GHB in dentate gyrus (DG) granule cell (n = 6). Grey area shows SEM. Left inset, representative image of a dentate gyrus granule cell. Scale bar 50 µm. Right inset, voltage response of a representative dentate gyrus granule cell to current injection. Scale bar 20 mV 200 ms. B2, Group results showing the mean holding current before and after GHB. B3, THIP induced a significant inward current (control: −131±10 pA THIP: −211±22 pA, n = 7, P = 0.01). C1, Mean holding current over time in response to the application of GHB in striatal medium spiny neurons (MSN) (n = 9). Grey area shows SEM. Left inset, representative image of a medium spiny neuron. Scale bar 50 µm. Right inset, voltage response of a representative medium spiny neuron to current injection. Scale bar 20 mV 200 ms. C2, Group results showing the mean holding current before and after GHB. C3, THIP induced a significant inward current (control: −314±26 pA THIP: −457±26 pA, n = 10, P<0.0001).