Table 1. Chicoric acid-dependent lifespan modulation of C. elegans.
Strain | Treatment | 50th | Mean lifespan | Max lifespan | Codeb | Statistics | N |
percentilea | days ± SD | days | (KM analysis)c | (censored)d | |||
N2 (wt) | Control | 19 | 17.94±3.87 | 24 | A | P = 0.0223 (B) | 120 (11) |
P = 0.0150 (C) | |||||||
P = 0.0182 (D) | |||||||
P<0.0001 (E) | |||||||
P<0.0001 (F) | |||||||
N2 (wt) | 5 µM CA | 20 | 18.6±4.46 | 24 | B | P = 0.0223 (A) | 60 (13) |
P = 0.8463 (C) | |||||||
P = 0.9821 (D) | |||||||
P = 0.0464 (E) | |||||||
P = 0.0002 (F) | |||||||
N2 (wt) | 10 µM CA | 20 | 18.52±4.57 | 25 | C | P = 0.0150 (A) | 62 (6) |
P = 0.8463 (B) | |||||||
P = 0.8808 (D) | |||||||
P = 0.0588 (E) | |||||||
P = 0.0003 (F) | |||||||
N2 (wt) | 25 µM CA | 20 | 18.73±4.35 | 24 | D | P = 0.0182 (A) | 53 (4) |
P = 0.9821 (B) | |||||||
P = 0.8808 (C) | |||||||
P = 0.0539 (E) | |||||||
P = 0.0003 (F) | |||||||
N2 (wt) | 50 µM CA | 20 | 20.16±3.76 | 26 | E | P<0.0001 (A) | 60 (4) |
P = 0.0464 (B) | |||||||
P = 0.0588 (C) | |||||||
P = 0.0539 (D) | |||||||
P = 0.0657 (F) | |||||||
N2 (wt) | 100 µM CA | 22 | 21.64±3.76 | 26 | F | P<0.0001 (A) | 60 (2) |
P = 0.0002 (B) | |||||||
P = 0.0003 (C) | |||||||
P = 0.0003 (D) | |||||||
P = 0.0657 (E) |
The tabulated data show the average results plotted in Figure 8. Up to two independent trials were done at 20°C with identical results. Worms were fed with the E. coli HT115 strain bacteria (see Material and Methods section). XLSTAT-life statistical software (Addinsoft, New York, NY, USA) was used to plot survival data by the Kaplan Meier method and differences between survival curves calculated using the Log-Rank test with 95% confidence. (a) Represents the 50th percentile (the age when the survival fraction of animals reaches 0.50). (b) Experiment identification code. (c) Probability of being identical to other lifespan experiments given in parentheses. (d) Total death scored (number of censored values).