Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Nov 11;203(3):457–470. doi: 10.1083/jcb.201302092

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Mosammaparast et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — LSD1 recruitment to sites of DNA damage is dependent on RNF168. (a) Flag-HA-LSD1 was stably expressed in U2OS cells, and then treated with the ATM inhibitor KU55933 (15 µM) or DMSO 1 h before laser microirradiation. The cells were then stained for HA and pH2A.X. (b) Wild-type (H2A.X^+/+) or H2A.X-deficient (H2A.X^−/−) MEFs stably expressing HA-tagged LSD1 were laser microirradiated and stained as in panel a. (c) Quantitation of a and b, with error bars representing the SD of duplicate experiments. (d) Wild-type or 53BP1-deficient (53BP1^−/−) MEFs stably expressing Flag-HA–tagged LSD1 were laser microirradiated and stained as in panel a. (e) U2OS cells were treated with the indicated shRNAs, microirradiated, and stained for endogenous LSD1 and pH2A.X. (f) Quantitation of d and e, with error bars representing the SD of duplicate experiments. Bars, 20 µm.