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. 2013 Nov 11;203(3):457–470. doi: 10.1083/jcb.201302092

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© 2013 Mosammaparast et al.

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Figure 4. — Transient retention of LSD1 and a rapid, H2A.X-independent recruitment of RNF168 to sites of DNA damage. (a) U2OS cells were laser microirradiated, incubated for the indicated time at 37°C, and fixed and stained for LSD1 and pH2A.X. (b) Quantitation of panel a. Error bars indicate ± SD of duplicate experiments. (c) Wild-type (H2A.X^+/+) or H2A.X-deficient (H2A.X^−/−) MEFs expressing GFP-tagged RNF168 were laser microirradiated, incubated for 10 min at 37°C, and fixed and stained as indicated. (d and e) Cells from panel c were exposed to 5 Gy IR, incubated at 37°C for the indicated times, and stained as in c. Bars, 20 µm.