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. 2013 Nov 11;203(3):417–425. doi: 10.1083/jcb.201303071

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© 2013 Tateishi et al.

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Figure 4. — Immunofluorescence microscopic images of centriole appendages in the DA+SA+, DA-SA−, and DA+SA− patterns. (A) Immunofluorescence for ninein, centriolin, CEP164, and OFD1. WT, (DA+SA+) centriole in WT F9 cells; KO, (DA-SA−) centriole in Odf2-KO F9 cells; Δ4/5, (DA+SA−) centriole in Δ4/5 construct–expressing Odf2-KO F9 cells; Δ6/7, (DA+SA+) centriole in Δ6/7 construct–expressing Odf2-KO F9 cells. (B) Schematic drawing showing the localization of centrosomal components. MC, mother centriole; DC, daughter centriole. Bars, 500 nm.