Figure 6.

Effect of antioxidant treatment on RILI in mif^−/− mice. Irradiated (20 Gy), aged (16-mo-old) mif^−/− and WT mice were treated with the antioxidants glutathione ester (GSH, 15 nM/kg) or N-acetyl cysteine (NAC, 100 mg/kg), starting 1 hour after irradiation via intraperitoneal injection, three times per week, for 4 weeks. RILI was attenuated in mif^−/− mice pretreated with GSH, although NAC pretreatment was not protective, as assessed by BAL fluid cell counts (A) and total protein concentrations (B), as well as inflammatory changes evident on lung histology (C; n = 5/group). *P < 0.05, compared with respective uninjured control mice. ^#P < 0.05, compared with respective irradiated control mice.