Figure 6.

Ephrin-B2 reverse signaling mediates distal lung fibrillar fibronectin (FN) deposition. Insoluble fibrillar FN expression was markedly increased in immunofluorescent analyses of distal alveoli cell–cell junctions (in B and D, arrows identify the insoluble fibrillar FN that is laid down as strands of FN; Cy3 was the secondary antibody, and 4′6-diamidino-2-phenylindole [DAPI] denotes a nucleus) and immunoblotting (E and F, equal protein loading, and soluble Fn serves as loading control) in 8-week-old Efnb2^LacZ/6YFΔV mice compared with WT mice (A, arrows demonstrate minimal fibrillar FN; C, immunofluorescence; E and F, Western Blot analysis). In addition, α5 integrin expression was markedly increased (G and H, P = 0.01) in Efnb2^LacZ/6YFΔV mice, compared with WT control mice (G and H). Magnification in A–D, scale bar = 20 μm.