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. 2013 Oct;49(4):525–535. doi: 10.1165/rcmb.2013-0019OC

TABLE 2.

EFFECTS OF MWCNT EXPOSURE AND OVA SENSITIZATION ON PROSTANOID CONCENTRATIONS DETECTED BY LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROSCOPY IN BRONCHOALVEOLAR LAVAGE FLUID FROM WILD-TYPE AND COX-2−/− MICE

Genotype Pretreatment OPA PGE2
 6-keto PGF1α
 PGF2α
 TXB2
 PGD2
1 d 14 d 1 d 14 d 1 d 14 d 1 d 14 d 1 d 14 d
WT
 Saline
 Vehicle
 47 ± 16
 38 ± 6
 11 ± 5
 4 ± 2
 17 ± 1
 10 ± 1
 21 ± 7
 7 ± 1
 107 ± 26
 72 ± 15
 
 Saline
 MWCNT
 56 ± 14
 59 ± 4
 11 ± 7
 7 ± 2
 12 ± 2
 13 ± 1
 14 ± 3
 11 ± 1
 72 ± 11
 85 ± 6
 
 OVA
 Vehicle
 88 ± 13
 91 ± 7
 16 ± 5
 6 ± 1
 39 ± 8
 26 ± 5
 102 ± 28*
 42 ± 13
 106 ± 32
 118 ± 23
 
 OVA
 MWCNT
 161 ± 68
 85 ± 12
 39 ± 16*
 6 ± 1
 49 ± 16
 15 ± 1
 63 ± 20
 25 ± 7
 96 ± 32
 131 ± 19
COX-2−/−
 Saline
 Vehicle
 76 ± 9
 60 ± 8
 12 ± 5
 10 ± 4
 20 ± 4
 13 ± 2
 26 ± 6
 17 ± 5
 106 ± 17
 120 ± 25
 
 Saline
 MWCNT
 98 ± 12
 91 ± 32
 14 ± 5
 11 ± 8
 19 ± 2
 18 ± 3
 24 ± 4
 23 ± 3
 137 ± 20
 170 ± 12*
 
 OVA
 Vehicle
 94 ± 43
 63 ± 10
 5 ± 1
 3 ± 1
 30 ± 8
 13 ± 1
 38 ± 12
 21 ± 9
 169 ± 104
 112 ± 10
  OVA MWCNT 175 ± 42 62 ± 7 9 ± 3 7 ± 3 66 ± 13 14 ± 1 120 ± 36* 20 ± 1 227 ± 97 87 ± 11

Definition of abbreviations: COX-2, cyclooxygenase-2; d, days; MWCNT, multiwalled carbon nanotubes; OPA; oropharyngeal aspiration; OVA, ovalbumin; PGD2, prostaglandin D2; PGE2, prostaglandin E2; PGF2α, prostaglandin F2α; 6-keto PGF1α, 6-keto prostaglandin F1α; TXB2, thromboxane B2; WT, wild-type.

Data are expressed as pg/ml (mean ± SEM). The vehicle consisted of saline/0.1% pluronic solution.

*

P < 0.05, compared with the corresponding genotype group.