Fig. 3.

mbd2/3(RNAi) Leads to disruption of tissue homeostasis. Most mbd2/3(RNAi) animals start to regress anterior regions by 2 weeks varying from mild to more extreme phenotypes (A). Head regression correlates with loss of cephalic ganglia (B) and the anterior gut branches (C) and (D). At 3 weeks almost all animals have completely lost anterior regions (red arrow-head), although a few display only mild anterior regression (E). By three weeks posterior gut branches have also started to be lost (F)–(H). While gut branches (blue arrows in (G)) and the pharynx (stars in (H) and (J)) are lost, the VNCs (white arrows in (J)) are maintained (F)–(H) and (J). Animals at 4 weeks have regressed further, but are mostly still alive (I). Scale bars 500 µm, except in C and G 200 µm. Panels in G represent regions in white boxes depicted in panel F.