Fig. 7.
Lack of evidence for a role for DNA methylation in pASC biology Smed-dnmt-2 is expressed in the germ line and germ line stem cells (A) Scale bars, 500 µm. gDNA digestion with the methylation dependent restriction enzyme McrBC does not indicate any DNA cytosine methylation in the Schmidtea mediterranea genome as compared with that of humans. −: no enzyme; +: with enzyme (B). Control chromatogram after HPLC-MS displaying the sensitivity of nucleosides detection, in particular for 5mC. (C) A representative chromatogram of S. mediterranea nucleoside analysis indicating the lack of 5mC (D).