Fig. 1.

Schematic diagram of the experimental procedures. (A) To investigate the effect of hippocampal cell survival on cells undergoing division prior to an inflammatory response, mice (n = 10 per group) were injected with BrdU (50 mg/kg) at P8 and then injected with saline or LPS (1 mg/kg). Thirty-three days later, animals were perfused and brains removed for immunohistochemistry. (B) To investigate the effect of hippocampal cell survival on cells undergoing division during an inflammatory response, mice (n = 10 per group) were injected with saline or LPS (1 mg/kg) at P9 and then injected with BrdU (50 mg/kg) at P11. Thirty days later animals were perfused and brains removed for immunohistochemistry. (C) To investigate the effects of LPS on hippocampal progenitor proliferation, mice (n = 10 per group) were injected with saline or LPS (1 mg/kg) at P9 and then injected with BrdU (50 mg/kg) at P11. Two hours after injection of BrdU the animals were perfused and brains removed for immunohistochemistry. (D) To investigate the effect of hippocampal cell survival in the dorsal respectively ventral horn, mice were injected with saline (n = 10) or LPS (1 mg/kg, n = 13) at P9 and then injected with BrdU (50 mg/kg) at P11. Forty-nine days later animals were perfused and brains removed for immunohistochemistry.