Figure 2.

Results of Increased Sequencing of Samples M4 and NA40

(A) Yield of unique fragments for M4 (Bronze Age hair) precapture (blue) and postcapture (red) libraries with increasing amounts of sequencing. The fold enrichment in number of unique reads with increasing amounts of sequencing is plotted in green, with values on the secondary y axis.

(B) Yield of unique fragments for NA40 (Peruvian bone) precapture (blue) and postcapture (red) libraries with increasing amounts of sequencing. The fold enrichment in number of unique reads with increasing amounts of sequencing is plotted in green, with values on the secondary y axis.

(C) Venn diagram showing the overlap between the NA40 pre- and postcapture libraries based on sequencing of 12.3 million reads.

(D) Coverage plot of the M4 and NA40 libraries based on sequencing of 18.6 million and 12.3 million reads, respectively. Shown is a random 10-megabase segment of chromosome 1. Coverage was calculated in 1 kb windows across the region.

(E) Insert size distribution for NA40 pre- and postcapture libraries.

(F) Percent GC content of reads for NA40 pre- and postcapture libraries.