Figure 2.

Suppression of scrib and puf60 in Zebrafish Leads to Short Stature, Small Head Size, and Craniofacial Defects

(A) Quantification of total body length was performed in embryo batches injected with sham (control), scrib MO, puf60a MO, and double MOs. Bars represent the mean length of 80 embryos at 3 dpf per condition, which were scored blind to injection cocktail.

(B) Lateral and dorsal views of representative control embryos and embryos injected with scrib or puf60a MO at 5 dpf; yellow line indicates the distance across the convex tips of the eye cups. Right panel, ventral views of corresponding embryos stained with Alcian blue at 5 dpf to visualize cartilage structures; yellow line indicates distance between ceratohyal (CH) and Meckel’s cartilages (MK).

(C and D) Quantification of head size (C) and craniofacial defects (D) was performed in control and embryo batches injected with scrib or puf60a MO by measuring distances as shown by the yellow lines in (B). Head size measurements are represented as a normal probability distribution curve in which the y axis represents the probability that the values of x fall within a certain interval.

(C) Significant differences were observed for the microcephaly phenotype; p < 0.0001 between control and puf60a morphants and p < 0.0001 between control and scrib morphants (three independent experiments; two-tailed t test comparisons).

(A and D) Data are shown as the standard error of the mean, SEM from three independent experiments, n = 80 embryos. The corresponding p values are denoted on the bar graphs (two-tailed t test comparisons).