Figure 3. Identification of the TgRON4-binding region in TUBB2C.

(a), (b) Upper: Schematic representation of TUBB2C and its N- and C-truncated mutants. Lower: The indicated HA-tagged TUBB2C proteins were coexpressed with 3xFLAG-tagged TgRON4B. The cell lysates were immunoprecipitated with an anti-HA antibody. The cell lysates and immunoprecipitates (IP) were analyzed by Western blotting with anti-HA and anti-FLAG antibodies. Owing to low expression, transient transfection of TUBB2C FR3 was scaled up (see Methods).