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. 2013 Nov 12;3:3194. doi: 10.1038/srep03194

Figure 1. Genetic variation in copy numbers of TaITS2 per genome among 20 wheat genotypes.

Figure 1

Total root DNA was isolated from primary roots of one month-old plants grown in a potting mix, and used to determined copy numbers of TaITS2 per genome using quantitative real-time PCR. Ct values of TaITS2 and TaPHT1;6A using sequence-specific primers and probes were determined for all samples. The copy number of TaITS2 per genome was calculated by 2ΔCt (ΔCt = TaPHT1;6A Ct – TaITS2 Ct). Predicted means of four biological replicates are presented.