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. 2013 Jul 24;33(11):1761–1769. doi: 10.1038/jcbfm.2013.124

Figure 2.

Figure 2

Effect of tissue-type plasminogen activator (tPA) on neuronal pAMPK expression. (A) Representative western blot analysis of adenosine monophosphate-activated protein kinase (AMPK) phosphorylated at Thr172 (pAMPK) in wild-type (Wt) cerebral cortical neurons incubated 0 to 60 minutes with 5 nmol/L of tPA. (B) Representative western blot analysis of pAMPK expression in the ischemic tissue of T4 mice and their Wt littermate controls 0 to 30 minutes after middle cerebral artery occlusion (MCAO). (C) Representative western blot analysis of pAMPK expression in the ischemic tissue of Wt mice treated with recombinant tPA (rtPA) immediately before 5 minutes of MCAO.