Figure 1.

Indirect immunofluorescence labelling of sections through tobacco seeds with Calcofluor White, LM6 arabinan and LM21 heteromannan probes. In some cases, the equivalent sections were pre-treated with endo-β–mannanase (+EβM) to remove the abundant heteromannan. E, embryo; ME, micropylar endosperm; NME, non-micropylar endosperm. White dashed lines with an asterisk indicate that a tissue asymmetry is detected. Brown dashed lines indicate no tissue level asymmetry was detected. Scale bar = 50 μm.