Fig. 3.

The observed antitumor effect of rAAV-SLC was immune-mediated. a The collected cells from tumor samples were stained FITC-CD11c, FITC-CD3 together with PE-CD4 or PE-CD8 for flow cytometric evaluation. Compared to control and rAAV-GFP, significantly higher numbers of CD11c+dendritic cells, CD4+T cells and CD8+T cells infiltration in SLC-treated mice were observed. Bars SD, *p < 0.05, **p < 0.01; i.t., intra-tumor injection; b serial 5 μm-thick Cryostat sections of tumor samples were performed by incubation with mAbs recognizing CD11c+dendritic cells, CD4+T cells and CD8+T cells. Positive cells were detected by DAB staining