Fig. 2.

Deletion of PEX5 by the Euroscarf strategy affects neighboring genes. (A) Genomic region of the PEX5 gene. The distance between PEX5 and PRP28 or MNN10 is 277 and 397 bps, respectively. Via the Euroscarf strategy, each gene was deleted from start to stop codon irrespectively of the genomic environment. A new strategy was designed to delete PEX5 from +250 to +1700 leaving 557 and 546 bps for the PRP28 and MNN10 promoters, respectively. (B) Growth curves of different yeast strains on media containing 0.5% glucose. The optical density was determined as absorbance at 600 nm. Data represent mean ± SEM, from at least two experiments. (C) Chronological lifespan of Δpex5 and Δ3'pex5 cells using WT as control. Data represent mean ± SEM from at least three experiments. (D) The mean and maximal chronological lifespans of Δpex5 and Δ3'pex5 cells calculated from the data shown in panel C. **P < 0.01.