Figure 10.

Distribution of GFP, tdTomato and synaptophysin staining at TC synapses. (A) Three-color image of an example TC synapse (image from a single section; green is post-synaptic GFP, red is pre-syanptic tdTomato, blue is pre-synaptic synaptophysin stain). (B) Pixel intensity map for each color channel for the single image shown in (A) (left) and the plot of pixel intensity averaged across all pixels vertically (right). (C) Pixel intensity map for each color channel averaged for 30 TC synapses. Individual images of synapses were first aligned based on PSDs of the correlative EM images and then cumulative signal intensity calculated. Note that the synaptophysin signal is skewed toward the presynaptic membrane whereas the tdTomato signal is not. Images shown are ~1.1 × 0.4 μm².