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. 2013 Nov 12;7:177. doi: 10.3389/fncir.2013.00177

Figure 2.

Figure 2

Large-scale array tomography images from barrel cortex of Thy1-YFPh mouse. (A) Maximum intensity projected YFP image from the aligned image stack obtained from 1074 serial sections. (B) Maximum intensity projection of 300 serial sections (=60 μm thick) from the box in (A) showing YFP-expressing L5 pyramidal neurons. (C) Orthogonal y-z view of (B). (D) Close up of box in (B), 150 serial sections (=30 μm thick) showing VPm axons/terminals in red, YFP-expressing L5 dendrites in green and synaptophysin staining in blue. (E) The tracings of the eight reconstructed L5 neurons superimposed on the max projection YFP image. Scale bars: 100 μm (A), 50 μm (B), 20 μm (D), 200 μm (E).