Figure 3.

TC synapse detection by AT. High power view of a section of dendrite of an YFP-expressing L5 pyramidal neuron with four example thalamo-recipient spines indicated (left panel; 3-D volume rendering of 13 serial sections = 2.6 μm thick). Right panels: images from three serial sections showing spine (YFP signal, green), TC terminal (tdTomato, red) and synaptophysin staining (blue) for each of the four indicated example TC synaptic contacts. Yellow arrowheads indicate locations of identified synaptic contacts (note that for synapses 2 and 4, the synaptic contact is detected in two of the images).