Table 1.
Chemicals used in the experiment.
| Chemicals (batch number) | Manufacturer |
|---|---|
| 50% Ortho-Phosphoric acid. (0000124351) | Panreac Química, Barcelona, Spain |
| Ethylenediaminetetreaacetic acid disodium salt-2-hydrate. (44006RLS) | |
| Clearfil™ SE Primer: HEMA 10-30%; 10-Methacryloyloxydecyl dihydrogen phosphate; Hydrophilic aliphatic dimethacrylate; dl-Camphorquinone; Water; Accelerators; Dyes; others. (01352A) | Kuraray Medical Inc., Tokyo, Japan |
| Xeno V: Bifunctional acrylate; acidic acrylate; functionalized phosphoric acid ester; acrylic acid; water, terciary butanol; initiator; stabilizer; Acetone<50%; UDMA<15%; Dipentaeryhritol pentaacrylate phosphate <15%; DMA <10%; TMA <10%; Other DMA <10% (0812000831) | Dentsply DeTrey, Konstanz, Germany |
| 0.9% Sodium chloride (5211B1) | Braun Medical, Barcelona, Spain |
| 20 % Chlorhedixine digluconate (9418600021) | Guinma, Valencia, Spain |
| Anhydrous calcium sulphate, Drierite, 6 mesh (14796TJ) | Sigma Aldrich, St. Louis, MO, USA |
| Borate -di-Sodium tetra-borate anhydrous- (0000173320) | Panreac Química, Barcelona, Spain |
| Brij® (35 0000173320) | |
| Calcium chloride anhydrous (0000208286) | |
| Glycerol (0000200971) | |
| HEPES buffer, Sterile, pH 8.0 −1 M- (8D007674) | Applichem, Darmstadt, Germany |
| MMP-2 active, Recombinant CHO cells −0.1 mg/mL- (D00065439) | Calbiochem, Gibbstown, NJ,USA |
| Penicillin G potassium salt (1386789) | Sigma-Aldrich, St. Louis, MO, USA |
| Sodium chloride 2-hydrate powder (0000159010) | |
| Streptomycin sulphate salt (026K8901) | |
| Zinc chloride (0000144046) |