Fig. 5.

Both wild-type human seipin and T78A mutant seipin form dodecamers. (a) AFM images of wild-type seipin either alone or after incubation with anti-Myc or anti-HA antibodies. (b) AFM images of T78A mutant seipin either alone or after incubation with either anti-Myc or anti-V5 antibodies. Arrows indicate multiply decorated seipin complexes. Scale bar, 100 nm; shade-height scale, 0–5 nm. (c, d) Gallery of zoomed images showing multiply decorated wild-type (c) or T78A mutant seipin (d). Scale bar, 20 nm; shade-height scale, 0–5 nm. (e, f) Frequency distributions of angles between pairs of antibodies bound to either wild-type (e) or T78A mutant seipin (f). The curves indicate the fitted Gaussian functions