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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Cancer Res. 2013 Aug 19;73(19):10.1158/0008-5472.CAN-13-0926. doi: 10.1158/0008-5472.CAN-13-0926

Figure 5. Obesity-like stromal cells accelerate tumorigenesis.

Figure 5

(A) Tumors from glands from NOD/SCID mice humanized with SVF/CCL2 cells developed earlier than those from SVF/EV humanized glands. Human mammary epithelial cells (HMEC) were isolated from 3 reduction mammoplasty tissue samples, transduced with lentiviruses encoding SV40er and KrasG12V, and transplanted into humanized glands co-mixed with either SVF/CCL2 or SVF/EV cells. (B) Tumors from SVF/CCL2 humanized glands were significantly larger in size and weight at four weeks after transplantation. N=6 tumors/group from 3 experiments with separate patient tissue samples (Table S1). (C) Tumors arising in SVF/CCL2 glands demonstrated significantly increased numbers of mitotic figures and Ki67 expression compared with those from SVF/EV glands. Mitotic figures and Ki67+ cells were quantified from 3 images from 12 tumors/group. Statistical differences were detected by t-test. (D) At 2 weeks following transplantation of transduced HMECs, glands humanized with SVF/CCL2 cells demonstrated significantly increased F4/80+ macrophage recruitment and increased CD31+ cells (n=6 tumors/group). Original magnification (C) 600x, 200x; (D) 200x; bar=100μm.