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. 2013 Nov 5;105(9):2055–2063. doi: 10.1016/j.bpj.2013.08.031

Figure 2.

Figure 2

Detailed characterization of GR-D121T and GR-H87M in oocytes. (A) Representative GR-D121T-mediated transient inward-directed photocurrents measured at pHo 7.5 and holding voltages between −125 mV and +75 mV in 25mV steps. (B) Resulting I(E)-Plot for GR-D121T (mean ± SE, n = 7). (C) Typical photocurrents of a GR-H87M expressing oocytes at different pHo and membrane voltages from −125 mV up to +50 mV in 25-mV steps. (D) I(E)-plot for stationary currents of GR-H87M (mean ± SE, n ≥ 9). (E) Action spectra for stationary currents of GR-H87M at different pHo and voltages as measured for GR-WT (compare to Fig. 1D). (Inset) Action spectra maxima (mean ± SD, n ≥ 6).