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. 2013 Nov 5;105(9):2055–2063. doi: 10.1016/j.bpj.2013.08.031

Figure 3.

Figure 3

Characterization of GR-WT and GR-H87M expressed in HEK cells. (A and D) Confocal images of transfected HEK-293 cells. ECFP was monitored one day after transfection. Scale bar: 10 μm. (B) Typical photocurrents of GR-WT are shown. Photo activation was achieved with light of 540 ± 7 nm (scale bar: 100 pA and 50 ms). (C and F) Action spectra for GR-WT peak currents (C) and GR-H87M stationary currents (F) measured at intracellular pHi 7.2 (black) and 4.0 (red), while pHo was kept at 7.2 and 0 mV membrane potential. Only mean values are shown to visualize spectral shifts. Note that deviations were similar spectral measurements in oocytes (GR-WT, n ≥ 8; GR-H87M, n = 5). (E) Typical photocurrents of GR-H87M. Photo activation was achieved with light of 520 ± 7 nm (scale bar: 100 pA and 50 ms). (G) Comparison of action spectra maxima from panels C and F (mean ± SD, n ≥ 5).