Figure 1. Schematic representation of prostate non-tumor and tumor endothelial cell isolation and enrichment.

Clinically examined and representative samples were mounted in OCT. Prostate tissues obtained from robotic radical prostectomy specimens from each patient were macrodissected for prostatic adenocarcinoma (tumor) and matched histologically benign regions. Portion of freshly macrodissected prostate tissues (tumor and non-tumor) were immediately digested and cultured in endothelial cell selection medium. Given that enough tissues were obtained, a portion of the tissues used for ECs isolation were embedded in OCT for frozen sections. ECs were isolated by CD31 magnetic Dynabead® and further enriched by CD31 fluorescent activated cell sorting (FACS).