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. 2013 Aug 11;4(9):1416–1426. doi: 10.18632/oncotarget.1217

Figure 1. ΔNp63 expression is elevated in cancer cells carrying an inactivated FA pathway/ inactivated FANCD2 under normoxic and hypoxic conditions.

Figure 1

Sets of HCT116, U2OS, and HTB-4 stably-transfected cell pairs isogenic to the status of the FA pathway were used to detect ΔNp63 mRNA and protein levels. Levels of both ΔNp63 mRNA and protein are higher in cells carrying an impaired FA pathway compared to the corresponding empty vector-containing control cells (carrying an intact FA pathway) under normal growth conditions or the treatment with 280 μM of Deferoxamine (DFO, a hypoxia-mimicking drug). (A) HCT116 colon cancer and (B) Human osteosarcoma U2OS cells stably express a down-regulated FANCL (Supplementary Figure 1). (C) HTB-4 bladder cancer cells stably express a higher level of FAVL and carry an impaired FA pathway [18]. (NIH Image J software was used to evaluate the band densities, with which the ΔNp63 expression levels were calculated upon the corresponding control =1).