Table 1. Effect of the TOR, PI3K and NF-κB inhibitors on the lifespan, fertility and locomotor activity Drosophila melanogaster.
| Compound (concentration) | Target | Lifespan | LA | F | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ♂ | ♀ | ♂ | ♀ | ♀ | ||||||
| M | 90% | n | M | 90% | n | |||||
| Rapa (0.005 μM) | TOR | ↑ (+14%)** | ↑(+4.7%) | 344 | ↑(+12%)** | ↑(+4.8%) | 363 | ↑ | ↑ | = |
| Wm (0.005 μM) | PI3K | ↑(+8%) | ↑(+4.7%) | 312 | ↑(+8%) | ↑(+4.8%) | 355 | ↑ | = | = |
| Wm (5 μM) | PI3K | ↑ (+5%)* | ↑ (+2%) | 259 | ↓ (-8.2%)* | ↓ (-1.7%) | 252 | ↑ | = | = |
| PDTC (1.25 μM) | NF-κB | ↑(+6%)* | ↑(+2%) | 437 | ↓(-2%)* | ↓(-5%)* | 451 | ↑ | = | = |
| PDTC (12.5 μM) | NF-κB | ↑(+7%)** | ↑(+6%)** | 448 | ↓(-2%)* | = | 433 | = | = | ↑ |
| PDTC (125 μM) | NF-κB | ↑(+10%)* | ↓(-3.1%)* | 322 | ↑(+12%)* | ↑(+4.8%) | 361 | = | = | = |
| QNZ (0.03 μM) | NF-κB | ↑(+2%) | ↑(+4%) | 297 | ↓(-15%)** | ↓(-4%)** | 298 | = | ↑ | ↑ |
| QNZ (0.3 μM) | NF-κB | = | ↑(+8%) | 279 | ↓(-4%) | ↓(-4%)** | 290 | ↓ | = | ↑ |
| QNZ (3 μM) | NF-κB | ↑(+2%) | = | 282 | ↓(-4%)* | ↓(-4%)** | 312 | ↑ | = | ↑ |
| 1400W (0.03 μM) | iNOS | ↑(+3%)** | ↑(+13%)** | 396 | ↓(-5%)** | ↓(-5%)** | 437 | = | ↓ | ↑ |
| 1400W (0.3 μM) | iNOS | = | = | 419 | ↓(-2%)** | ↓(-5%)** | 453 | ↑ | ↑ | ↑ |
| 1400W (3 μM) | iNOS | ↑(+7%)** | ↑(+5%)* | 428 | ↓(-2%)** | ↓(-4%)** | 449 | ↑ | ↓ | = |
| Rapa (5 μM) + Wm (5 μM) | TOR + PI3K | ↑ (+2.4%)* | ↑ (+8%) | 372 | ↑ (+14.6%)* | ↑ (+23.4%)* | 400 | - | - | - |
| PDTC (125 μM) + Rapa (0.005 μM) | NF-κB + TOR | ↑(+10%)** | ↓(-3.1%) | 305 | ↑(+10%)* | ↑(+11.3%)** | 370 | ↑ | ↑ | = |
| PDTC (125 μM) + Wm (0.005 μM) | NF-κB + PI3K | ↑(+10%)* | ↑(+4.7%) | 304 | ↑(+12%)* | ↑(+8.1%)* | 342 | ↑ | ↑ | = |
Legend: «↑» – increasing, «↓» - decreasing, "=" – no effect "-" – not studied, M - median lifespan, 90% - the age of 90% mortality, n - sample size, LA - locomotor activity, F – fertility, *p<0.05, ***p<0.01 – Gehan–Breslow–Wilcoxon and Mantel–Cox tests for M, Wang-Allison test for 90%.