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. 2013 Aug 6;4(9):1399–1415. doi: 10.18632/oncotarget.1202

Figure 3. Ectopic c-Kit overrides miR-34a-dependent inhibition of Erk signaling and colony formation in soft agar.

Figure 3

(A) DLD-1/pRTR-pri-miR-34a cells were treated with DOX for the indicated time-points. The indicated proteins were detected by Western blot analysis. α-tubulin served as a loading control. (B) Western blot analysis of the indicated SW480 and DLD-1 cells after oligonucleotide transfection for 48 hours and addition of DOX for 24 hours. α-tubulin served as a loading control. le = long exposure, se = short exposure. (C) Soft agar colony formation assay. The indicated cells were treated as described under (B). Results represent the mean +/−S.D. (n=3) and significance was calculated applying a Student's t-test.” * “: p < 0.05.