Fig. 2.

Cardiomyocyte viability after ErbB2 inhibition. Fig a/b: Human cardiomyocytes were treated with doxorubicin (0–250 μg/ml) and trastuzumab (0–10 μg/ml) for 3 days. Cells were then washed (3× with PBS) to remove drugs and evaluated for cell viability directly (2a) or re-incubated with trastuzumab only until evaluation at 14 days (2b). Each symbol represents the mean (±SEM) of three experiments and results are expressed as percentage cell survival. Fig c/d: Human cardiomyocytes were irradiated (0, 2.5, 5, or 10 Gy) and then exposed to trastuzumab (0–10 μg/ml) for 14 days (c) or 21 days (d) before evaluation of cell viability. Each symbol represents the mean (±SEM) of three experiments and results are expressed as percentage cell survival