Table 2.
Primers used in this study
| Primer | Sequence 5′ → 3′ |
|---|---|
| 0183P1 | GGATCCTGAGGATAATCGCAAAG 1 |
| 0183P2 | AGATCTAGCAAGTGCAACAACAACCA 2 |
| M1 | TTTTTTCTTGTATTTTATAAGAAAAAACTAAGACTTATAAGAAATATTTTTCTC |
| M35 | AAATTTACTTTTAATTGTGTATAATAAGGGTTAATAATATTTTATTTCTTAAGG |
| M35-16 | GTATAATAAGGGTTAATAATATTTTATTTCTTAAGGAGCTTCATTG |
| M35-16-10 | AAGGGTTAATAATATTTTATTTCTTAAGGAGCTTCATTGG |
| Mrest-10 | GGATCCCACAATTAAAAGTAAATTTAATATTTGAATTTTTTCTTGTATTTTATAAGAAAAAACTAAGACTTATAAGAAATATTTTTCTC 1 |
| Mrest-16 | AGATCTGTAAATTTAATATTTGAATTTTTTCTTGTATTTTATAAGAAAAAACTAAGAC 2 |
| Mcons-16 | CCAAAAAAAAGTAAATTTAATATTTGAATTTTTTCTTGTATTTTATAAGAAAAAACTAAGAC |
| Mcons-35 | TTTAAGTATTAAATTTACTTTTAATTGTGTATAATAAGGGTTAATAATATTTTATTTCTTAAGG |
| 183-6FAM | CACACCATAGGCATAGAAT |
Underlined letters indicate restrictase recognition sequences introduced for cloning purposes: 1 BamHI and 2 BglII