Skip to main content
. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Vascul Pharmacol. 2013 May 30;59(0):10.1016/j.vph.2013.05.004. doi: 10.1016/j.vph.2013.05.004

Fig. 1.

Fig. 1

Agonist-stimulated dephosphorylation of eNOS at S116 and phosphorylation of eNOS at Y83 in BAECs. BAECs were either not treated (control) or treated for 10 min with bradykinin (BK, 1µM), thapsigargin (TG, 100 nM), ATP (10 µM), or angiopoietin (ANG, 50 ng/ml). Cells were lysed and equal quantities of lysate protein from each condition were immunoblotted (IB) with anti-phospho-S116 eNOS, anti-phospho-Y83 eNOS, and non-phospho-specific anti-eNOS antibodies, Results shown are representative of at least 3 separate experiments.