Effects of calcineurin inhibition on agonist-induced phosphorylation of eNOS at Y83 in BAECs. BAECs were either not pretreated or pretreated with: Panel A, cyclosporin A (CsA, 100 nM for 30 min), or Panel B, calcineurin autoinhibitory peptide (CAIP, 50 µM for 2 h) or a peptide in which the autoinhibitory sequence was scrambled (50 µM for 2h). Cells were then either not stimulated or stimulated with bradykinin (BK, 1 µM for 10 min). Cell lysates were immunoblotted (IB) with either anti-phospho-Y83 eNOS antibody or non-phospho-specific anti-eNOS antibody. Similar results to those shown were obtained in at least 3 separate experiments.