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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Vascul Pharmacol. 2013 May 30;59(0):10.1016/j.vph.2013.05.004. doi: 10.1016/j.vph.2013.05.004

Fig. 6.

Fig. 6

Effects of calcineurin inhibition on agonist-induced phosphorylation of eNOS at Y83 in BAECs. BAECs were either not pretreated or pretreated with: Panel A, cyclosporin A (CsA, 100 nM for 30 min), or Panel B, calcineurin autoinhibitory peptide (CAIP, 50 µM for 2 h) or a peptide in which the autoinhibitory sequence was scrambled (50 µM for 2h). Cells were then either not stimulated or stimulated with bradykinin (BK, 1 µM for 10 min). Cell lysates were immunoblotted (IB) with either anti-phospho-Y83 eNOS antibody or non-phospho-specific anti-eNOS antibody. Similar results to those shown were obtained in at least 3 separate experiments.