Figure 2.

Chemical denaturation and refolding kinetics of DS119. (a) Chemical denaturation curves measured by CD (red) and Trp fluorescence (black). (b) Chemical denaturation curves of different concentrations of DS119 measured by Trp fluorescence. (c) Stopped-flow refolding kinetics measured by CD. (d) Stopped-flow refolding kinetics measured by Trp fluorescence. In the kinetic experiments, the final concentration of Gdn-HCl was 0.75 M. Red lines show the fitting to a single-exponential function.