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. 2013 Oct 3;4(10):e828. doi: 10.1038/cddis.2013.337

Figure 5.

Figure 5

CD44+CD54+ R-CICs are resistant to chemotherapeutic agents and apoptosis in vitro. (a) Examination of k-ras gene mutation with sequencing, where codons 12 and 13 of exon 2 are wild type. One representative graph of three different tumors is shown. (b) Immunoblotting or immunohistochemical validation of EGFR expression in different cell populations derived from two independent experiments (patients 9 and 15). (c) Percentage of cell viability of different cellular subpopulations and caco-2 cells after treatment with 5-Fu, oxaliplatin, and cetuximab of different concentrations for 24, 48, and 72 h in vitro. Data are mean±S.D. of three independent experiments, each performed with cells from different donors (**P<0.01, *P<0.05; patients 9, 11, and 15). (d) Cell apoptosis percentage of different cellular subpopulations and caco-2 cells after treatment with 5-Fu, oxaliplatin, or cetuximab for 24, 48, or 72 h. Data are mean±S.D. of three independent experiments, each performed with cells from different donors (**P<0.01, *P<0.05; patients 9,11, and 15)