Figure 6.

p62-mediated cytoprotective ER stress is attributed to ERK1/2-dependent inhibitory BAD phosphorylation. (a) Regulation of PAT on BCL2 family proteins and ERK1/2. The cells were treated with various concentrations of PAT for 24 h and then the expression of BCL2, Bcl-xL, MCL1 and phosphorylation of ERK1/2 and BAD were analyzed by western blotting. (b) Effects of UPR inactivation by RNAi on PAT-induced phosphorylation of ERK1/2 and BAD. (c) Influences of p62 inhibition on PAT-induced phosphorylation of ERK1/2 and BAD