Figure 1.

miR-30e was induced during adipocyte formation and reduced during osteoblast differentiation. Real-time PCR showed that AIM treatment of marrow stromal cells for 72 h induced all miR-30 family members, whereas OIM reduced miR-30c, miR-30d and miR-30e levels and did not affect miR-30a and miR-30b (a–e). miR-30e was also induced in 3T3-L1 (f) and C3H10T1/2 (g) at 48 h after treatment with AIM. In contrast, miR-30e was reduced in a time-dependent manner in ST2 (h) and MC3T3-E1 (i) after treatment with OIM. Values represent the mean±S.D. of three experiments. *Significant versus Vehicle, P<0.05