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Inactivation of miR-30e reduced 3T3-L1 differentiation. The miR-30e inhibitor transfection blocked the adipocyte formation (a and b). Transfection of miR-30e inhibitor decreased the mRNA expression levels of PPARγ, C/EBPα, C/EBPβ and aP2 at all the indicated time points (c–f), and reduced protein levels of PPARγ, C/EBPα and aP2 at 72 h (g). Values represent the mean±S.D. of three experiments. *Significant versus control, P<0.05
