Figure 5.

Perturbation of miR-30e in MC3T3-E1 altered osteoblast differentiation. The miR-30e mimic transfection blunted ALP staining (a), decreased mRNA levels of Runx2, osterix, osteocalcin, ALP and BSP (b) and reduced protein levels of Runx2, osterix and ALP (c). In contrast, miR-30e inhibitor transfection enhanced ALP staining (d), induced mRNA levels of Runx2, osterix, osteocalcin, ALP and BSP (e) and increased protein levels of Runx2, osterix and ALP (f). Values represent the mean±S.D. of three experiments. *Significant versus control, P<0.05