Figure 7.

miR-30e directly targeted LRP6. The 3′-UTR fragments of Runx2, Wnt7b and LRP6 were PCR-amplified and cloned (a). The miR-30e miRNA overexpression in AD-293 slightly decreased the luciferase activity of Runx2 construct (b), whereas it had no impact on Wnt7b construct (c). The luciferase activity of LRP6 construct was dramatically decreased following miR-30e overexpression (d). Deletion of the miR-30e-binding sequence from the LRP6 3′-UTR construct increased the luciferase activity (e). Furthermore, miR-30e mimic transfection induced, and miR-30e inhibitor transfection reduced, LRP6 and β-catenin protein in 3T3-L1 cells (f and g). Values represent the mean±S.D. of three experiments. *Significant versus control, P<0.05