Figure 5.

Effect of LHX6 knockdown on cell growth. (a) LHX6 mRNA-targeting siRNA and a non-targeting control siRNA were transfected into LHX6-expressing HBE cells. The efficiency of LHX6 knockdown was examined by real-time RT-PCR. (b) siRNA-mediated knockdown of LHX6 induced cell proliferation as shown by CCK-8. (c) Colony formation assays confirmed the effect of LHX6 expression on cell growth. Colony numbers were increased by knockdown of LHX6. Colonies were photographed under a phase-contrast microscope (left, c1). Average numbers of colonies are represented by the bar graph (right, c2). Each experiment was repeated three times. (d) Cell cycle analysis by flow cytometry. The distribution of siRNA negative control- and LHX6 siRNA-transfected HBE cells in cell cycle phases. The percentages are represented by the bar graph. Each experiment was repeated three times. (e) The effect of LHX6 knockdown on apoptosis was analysed by flow cytometry. *P<0.05; **P<0.01