Figure 5.

Downregulation of TRAP1 sensitizes HCT116 cells to drugs targeting cap-dependent translation. (a, b) Rates of apoptotic cell death in HCT116 cells treated with Ribavirin (a) (100 or 200 μg/ml) or 4EGI-1 (b) (25 or 50 μM) for 48 h upon stable downregulation of TRAP1. All data are expressed as mean±S.D. from three independent experiments; *P≤0.01, **P≤0.001, ***P≤0.0001, n=3. (c) Representative images (left) of transwell migration assay using 10% FBS as chemoattractant. The data summary (right) shows absorbance of the Crystal violet staining eluted by migrated cells and quantitated by spectrophotometric reading at 570 nm. (d) TRAP1, eIF4A, eIF4E and eEF1G, eEF1A expression in human CRCs. Total cell lysates from four human CRCs (T) and the respective non-infiltrated peritumoral mucosas (M) (2 TRAP1-positive and 2 TRAP1-negative tumors) were separated by SDS-PAGE and immunoblotted using indicated antibodies