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. 2013 Oct 10;4(10):e852. doi: 10.1038/cddis.2013.381

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Copyright © 2013 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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Deregulation of T91/T93 phosphorylation recovers cell death in presence of lithium. (a and b) Confocal analysis of CGCs generated from c-Jun ΔN mice and infected with GFP-tagged lentivirus particles expressing c-Jun-wt-HA (a) or Ha-c-JunD95-HA proteins (b). After transduction, cells were cultured for 6 days and then treated as indicated with 25 or 5 mM K for 18 h, with or without SP600125 (10 μM) or LiCl (10 μM) as indicated. After fixation, nuclei were stained by DAPI and immunostained with HA antibody (red). (c) Quantification of results shown in a and b. Columns indicate the average percentage number of condensed nuclei present in 10 different fields, with bars indicating S.D.