Fig. 1.

Representative agarose gel electrophoresis of PCR amplicons. a GP5+/6+ PCR demonstrating ~140 base pair amplification from the HPV L1 open reading frame (ORF); amplicons of predicted size apparent in lanes a, d, f, g, i, j, and o. b pU1-M/pU-2R PCR demonstrating ~240 base pair amplification from the HPV E6/E7 ORF; amplicons of predicted size apparent in lanes a, b, c, e, g, i, l, m, o, q, r. c HPV-31 primer specific PCR demonstrating ~155 base pair amplification from the HPV E5 ORF; amplicons of predicted size apparent in lanes b, d, e, g, i, j, k, l, m, n, q. Confirmation of amplicon identity as HPV sequences by cycle sequencing (Fig. 2). M—50 base pair ladder; lower case letters: samples; 31—HPV-31 plasmid control; nc—negative controls