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. 2013 Jun 2;8(6):637–648. doi: 10.1007/s12263-013-0347-5

Table 1.

Sequences of the primers used for RT-qPCR

Genes Primer sequence (5′–3′) Annealing temperature (°C) Amplicon size (bp) References
TNF-α Sae F
TNF-α Sae R
agc cca tgt tgt agc aaa cc
tga ggt aca ggc cct ctg at
58 134 Saegusa et al. (2007)
IL-6 Tatjana F
IL-6 Tatjana R
ttc aat gag gag act tgc ctg
aca aca aca atc tga ggt gcc
60 349 Faruqi et al. (2001)
β-Actin F
β-Actin R
tgg ctg ggg tgt tga agg tct
agc acg gca tcg tca cca act
58 238 Lallemant et al. (2009)
TNF-α mus F
TNF-α mus R
tct cat cag ttc tat ggc cc
ggg act aga caa ggt aca ac
54 212 Dombrowicz et al. (2001)
IL-6 mus F
IL-6 mus R
gtt ctc tgg gaa atc gtg ga
tgt act cca ggt agc tat gg
54 209 Dombrowicz et al. (2001)
MCP-1 mus F
MCP-1 mus R
tct ggg cct gct gtt cac
gtg aat gag tag cag cag gtg agt
54 96 This study
VCAM-1MF1
VCAM-1MR1
tgg tga aat gga atc tga acc
ccc aga tgg tgg ttt cct t
54 86 This study
ICAM-1MF1
ICAM-1MR1
gga cca cgg agc caa ttt c
ctc gga gac att aga gaa caa tgc
55 82 This study
E-selectin MF
E-selectin MR
ccc tgc cca cgg tat cag
ccc ttc cac aca gtc aaa cgt
55 85 This study
ACTB mus2F
ACTB mus2R
agt gtg acg ttg aca tcc gta
gcc aga gca gta atc tcc ttc t
54 112 This study