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. 2013 Aug 3;30(10):1313–1318. doi: 10.1007/s10815-013-0041-y

Table 1.

Information of specific primers used in nested quantitative real-time PCR analysis

Gene Primer sequence (5′ → 3′) Size (bp) Accession no.
GDF9
  First Pair Forward CGTCCCAACAAATTCCTCCTT 176 NM_005260
Reverse AGGCCAGCTCTGTCTCTCTCAT
  Second Pair Forward CTGCTTTGCCTGGCTGTGT 105
Reverse CAAGGCATAGCCCCAGATTC
BMP15
  First Pair Forward ACCATGGTGAGGCTGGTGAA 179 NM_005448
Reverse ACATGGCAGGAGAGATTGAAGC
  Second Pair Forward GGCAAGGCCTCACAGAGGTA 102
Reverse CGGTAAACCACAGTGGCTCTAAC
β-actin
  First Pair Forward CTTACAGATCATGTTTGAGACCTTCAA 417 NM_001101
Reverse CTCAGGGCAGCGGAACC
  Second Pair Forward GCGCGGCTACAGCTTCA 59
Reverse TCTCCTTAATGTCACGCACGA T