Table 1.
Information of specific primers used in nested quantitative real-time PCR analysis
| Gene | Primer sequence (5′ → 3′) | Size (bp) | Accession no. |
|---|---|---|---|
| GDF9 | |||
| First Pair | Forward CGTCCCAACAAATTCCTCCTT | 176 | NM_005260 |
| Reverse AGGCCAGCTCTGTCTCTCTCAT | |||
| Second Pair | Forward CTGCTTTGCCTGGCTGTGT | 105 | |
| Reverse CAAGGCATAGCCCCAGATTC | |||
| BMP15 | |||
| First Pair | Forward ACCATGGTGAGGCTGGTGAA | 179 | NM_005448 |
| Reverse ACATGGCAGGAGAGATTGAAGC | |||
| Second Pair | Forward GGCAAGGCCTCACAGAGGTA | 102 | |
| Reverse CGGTAAACCACAGTGGCTCTAAC | |||
| β-actin | |||
| First Pair | Forward CTTACAGATCATGTTTGAGACCTTCAA | 417 | NM_001101 |
| Reverse CTCAGGGCAGCGGAACC | |||
| Second Pair | Forward GCGCGGCTACAGCTTCA | 59 | |
| Reverse TCTCCTTAATGTCACGCACGA T | |||